Journal: Nature Immunology
Article Title: PD-1 receptor deficiency enhances CD30 + T reg cell function in melanoma
doi: 10.1038/s41590-025-02172-0
Figure Lengend Snippet: a – g , Tumor-bearing Rag1 −/− mice were reconstituted with different T cell subsets and then subjected to CosMx spatial transcriptomics: UMAPs of 9,231 cells derived from WT T eff /WT T reg cell and WT T eff / Pd1 −/− T reg cell cohorts, color-coded by assigned cell type (left), and WT and Pd1 −/− T reg cell cohorts decoupled (middle and right) ( a ); frequencies of all cells in the TME ( b ); immune cells subclusters and frequencies of immune cell subsets among WT and Pd1 −/− T reg cells ( c ); a graphical representation of cell–cell interaction analysis (left) and numbers of cell–cell interactions of WT T reg cells (middle) and Pd1 −/− T reg cells (right) ( d ); a graphical representation of SR and LR gene definition by manual annotation (left), and frequencies of LR and SR interactions of WT (middle) and Pd1 −/− T reg cells (right) ( e ); and DC Il10 expression ( f ) and Gzmb expression in NK cells ( g ) according to clustered Wilcoxon rank-sum test are shown. h , i , Tamoxifen-treated, tumor-bearing Pd1 fl/fl and Pd1 fl/fl Foxp3 ERT2Cre mice were immunophenotyped on day 19: the frequencies of IL-10 expression in TIL DCs ( n = 5 per group) ( h ) and GzmB expression in TIL NK cells ( n = 10 for Pd1 fl/fl and n = 9 for Pd1 fl/fl Foxp3 ERT2Cre ) ( i ) are shown. j , For mice as in a – g , FOVs showing colocalization of different WT and Pd1 −/− T reg cell states and their interactions within the TME. k , l , Tamoxifen-treated, tumor-bearing Pd1 fl/fl Foxp3 ERT2Cre mice were immunophenotyped ( n = 8): representative flow cytometry results ( k ) and a summary of CD30 expression in T reg cell subsets ( l ) are shown. Data represent individual cells in f and g . In h , i and l , data are from three independent experiments, and the mean ± s.e.m. is shown. In h and i , each data point represents an individual mouse. In l , data points are matched within animals. Two-tailed clustered Wilcoxon rank-sum test was used for f and g, two-tailed unpaired Student’s t -test for h and i , and one-way ANOVA with Sidak’s multiple comparison test for l . * P < 0.05, ** P < 0.01. Illustrations in d and e created using BioRender.com . ILC, innate lymphoid cell.
Article Snippet: Fig. 5 Spatial transcriptomics of Pd1 −/− T reg cell communication programs in TME. a – g , Tumor-bearing Rag1 −/− mice were reconstituted with different T cell subsets and then subjected to CosMx spatial transcriptomics: UMAPs of 9,231 cells derived from WT T eff /WT T reg cell and WT T eff / Pd1 −/− T reg cell cohorts, color-coded by assigned cell type (left), and WT and Pd1 −/− T reg cell cohorts decoupled (middle and right) ( a ); frequencies of all cells in the TME ( b ); immune cells subclusters and frequencies of immune cell subsets among WT and Pd1 −/− T reg cells ( c ); a graphical representation of cell–cell interaction analysis (left) and numbers of cell–cell interactions of WT T reg cells (middle) and Pd1 −/− T reg cells (right) ( d ); a graphical representation of SR and LR gene definition by manual annotation (left), and frequencies of LR and SR interactions of WT (middle) and Pd1 −/− T reg cells (right) ( e ); and DC Il10 expression ( f ) and Gzmb expression in NK cells ( g ) according to clustered Wilcoxon rank-sum test are shown. h , i , Tamoxifen-treated, tumor-bearing Pd1 fl/fl and Pd1 fl/fl Foxp3 ERT2Cre mice were immunophenotyped on day 19: the frequencies of IL-10 expression in TIL DCs ( n = 5 per group) ( h ) and GzmB expression in TIL NK cells ( n = 10 for Pd1 fl/fl and n = 9 for Pd1 fl/fl Foxp3 ERT2Cre ) ( i ) are shown. j , For mice as in a – g , FOVs showing colocalization of different WT and Pd1 −/− T reg cell states and their interactions within the TME. k , l , Tamoxifen-treated, tumor-bearing Pd1 fl/fl Foxp3 ERT2Cre mice were immunophenotyped ( n = 8): representative flow cytometry results ( k ) and a summary of CD30 expression in T reg cell subsets ( l ) are shown.
Techniques: Derivative Assay, Expressing, Flow Cytometry, Two Tailed Test, Comparison
